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Comparative Study
. 1995 Feb;36(1):60-2.

Comparison of Pseudomonas pseudomallei from humans, animals, soil and water by restriction endonuclease analysis

E H Yap  1 , T W Thong, A L Tan, M Yeo, H C Tan, H Loh, T P Teo, K T Thong, M Singh, Y C Chan
Affiliations
  • PMID: 7570138
Comparative Study

Comparison of Pseudomonas pseudomallei from humans, animals, soil and water by restriction endonuclease analysis

E H Yap et al. Singapore Med J. 1995 Feb.

Abstract

Pseudomonas pseudomallei isolates from 62 human, 17 animal, 3 soil and 3 water samples were examined by genomic DNA digestion with PstI. Five major (RE I, II, III, IV, V) reproducible restriction patterns were observed, with most (56/62) of the human isolates displaying RE I (30/62), II (5/62), III (15/62), IV (4/62), V (2/62), and the animal (16/17), soil (2/3), water (3/3) isolates showing predominantly RE II profiles. Six human and one soil isolates showed patterns different from those of RE I to V. Restriction endonuclease analysis may be applied in epidemiological studies of melioidosis.

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