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Comparative Study
. 1976 Jun;3(6):599-603.
doi: 10.1128/jcm.3.6.599-603.1976.

Counterimmunoelectrophoresis of staphylococcal antibody

J Fedorko, B E Fossieck Jr, R H Parker
Comparative Study

Counterimmunoelectrophoresis of staphylococcal antibody

J Fedorko et al. J Clin Microbiol. 1976 Jun.

Abstract

Modifications of the staphylococcal counterimmunoelectrophoresis technique were evaluated to determine how variations in the procedure affected results. Neither a buffer pH range of 7.8 to 9.0 nor buffer molarity of 0.015 or 0.025 when tested in combinations caused appreciable differences. However, use of different agar preparations or delay in addition of antigen to the test slide altered the location of the precipitin band. Agarose was found to be more sensitive in determining the serum precipitin titer and provided a better photographic record than either Ionagar or Noble agar.

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References

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    1. J Immunol. 1974 Jun;112(6):2193-201 - PubMed
    1. J Infect Dis. 1973 Jan;127(1):106-9 - PubMed
    1. Ann Intern Med. 1975 Jan;82(1):13-7 - PubMed

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