Determination of furazolidone in porcine tissue using thermospray liquid chromatography-mass spectrometry and a study of the pharmacokinetics and stability of its residues

Abstract

A method is presented for the detection of the nitrofuran, furazolidone, in porcine tissue. Following methanol-buffer extraction of the tissue, liquid partitioning, and solid-phase clean-up, samples are analysed by using thermospray LC-MS monitoring the positive ion m/z 243 with filament-assisted ionization. The LOD is 1 microgram kg-1. The assay is used to investigate the depletion of furazolidone from tissue and sample stability post mortem. It is necessary to snap-freeze samples by immersion in liquid nitrogen immediately upon collection in order to improve the stability of residues in tissue.