Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Sep;61(9):3476-8.
doi: 10.1128/aem.61.9.3476-3478.1995.

Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water

C R Arias  1 , E Garay, R Aznar
Affiliations

Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water

C R Arias et al. Appl Environ Microbiol. 1995 Sep.

Abstract

A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.

PubMed Disclaimer

References

    1. J Mol Biol. 1981 May 15;148(2):107-27 - PubMed
    1. Appl Environ Microbiol. 1991 Mar;57(3):707-11 - PubMed
    1. Am J Epidemiol. 1991 Aug 1;134(3):290-7 - PubMed
    1. Appl Environ Microbiol. 1991 Sep;57(9):2640-4 - PubMed
    1. Appl Environ Microbiol. 1991 Sep;57(9):2651-5 - PubMed

Publication types

LinkOut - more resources