doi: 10.1128/aem.61.9.3476-3478.1995.
Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water
Affiliations
- PMID: 7574657
- PMCID: PMC167627
- DOI: 10.1128/aem.61.9.3476-3478.1995
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Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water
Appl Environ Microbiol.
1995 Sep.
Abstract
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
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