[Determination of hexokinase isoenzymes in different tissues]
- PMID: 758095
[Determination of hexokinase isoenzymes in different tissues]
Abstract
A modified method is described for estimation of hexokinase isoenzymes by polyacrylamide gel electrophoresis. The best conditions for detection of hexokinase activity were developed using immobilization of glucose-6-phosphate dehydrogenas: in gel, utilization of riboflavin as a catalyst of polymerization and stabilization by glucose. The method is simple, highly reproducible, comparatively rapid and economically reasonable.
Similar articles
-
[Detection of hexokinase isoenzymes on polyacrylamide gel using tetrazolium salts].Lab Delo. 1985;(7):441-2. Lab Delo. 1985. PMID: 2414535 Russian. No abstract available.
-
Characterization of some of the enzymes in ragweed pollen.Ann Allergy. 1976 Jun;36(6):410-8. Ann Allergy. 1976. PMID: 1275328
-
[Effect of cAMP on hexokinase and glucose-6-phosphate dehydrogenase isoenzymes in different white rat tissues].Biull Eksp Biol Med. 1981 Jul;92(7):52-4. Biull Eksp Biol Med. 1981. PMID: 6271287 Russian.
-
Isoenzyme analyses of Malaysian Schistosoma, S. mekongi and S. japonicum by isoelectric focusing in polyacrylamide gel.Southeast Asian J Trop Med Public Health. 1985 Dec;16(4):539-45. Southeast Asian J Trop Med Public Health. 1985. PMID: 2940688
-
Microelectrophoresis as a tool in enzyme histochemistry.Histochem J. 1981 Mar;13(2):207-25. doi: 10.1007/BF01006880. Histochem J. 1981. PMID: 7019163 Review. No abstract available.