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. 1995 Jul 21;669(2):265-9.
doi: 10.1016/0378-4347(95)00100-w.

On-line solid-phase extraction of ceftazidime in serum and determination by high-performance liquid chromatography

S Bompadre  1 , L Ferrante, F P Alò, L Leone
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On-line solid-phase extraction of ceftazidime in serum and determination by high-performance liquid chromatography

S Bompadre et al. J Chromatogr B Biomed Appl. .

Abstract

A column-switching high-performance liquid chromatographic assay is described for the determination of ceftazidime (a third-generation cephalosporin) in human serum. The method does not require prior sample pretreatment. Serum is directly injected in a first chromatographic column for sample clean-up and extraction. Thereafter, using an on-line column-switching system, the drug is quantitatively transferred and separated on a second, analytical column followed by determination using ultraviolet absorption at 258 nm. The technique allows direct, rapid, precise, and simple determination of ceftazidime in serum over the range of 1-250 micrograms/ml using 12.5 microliters of serum. This method was applied to study the pharmacokinetics of the drug in patients undergoing vascular surgery.

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