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Comparative Study
. 1995 Sep-Oct:(5):26-8.

[Development of a method of obtaining lyophilized antileukocyte histotyping sera intended for long-term storage]

[Article in Russian]
R P Ivanova, G A Kochetkova, N P Sivakova, L N Bubnova, A E Khoroshaĭlova
  • PMID: 7582770
Comparative Study

[Development of a method of obtaining lyophilized antileukocyte histotyping sera intended for long-term storage]

[Article in Russian]
R P Ivanova et al. Klin Lab Diagn. 1995 Sep-Oct.

Abstract

An optimal scheme for sublimation of anti-HLA-sera of locuses A, B, C, and DR has been developed, consisting of freezing at 60 degrees C and lyophilization to 29-30 degrees C, this permitting the preparation of initially active antileukocytic sera. The activity and specificity of defrosted native and lyophilized anti-HLA-sera were assessed in the complement-dependent cytotoxicity test. Four-year follow-up of the activity of dried anti-HLA-sera showed that the stabilizer was not needed, for the activities of the sera lyophilized with and without it was virtually equally high. The optimal storage temperature for dry anti-HLA-sera is 4 degrees C.

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