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. 1995 Aug;71(4):224-7.
doi: 10.1136/sti.71.4.224.

Asymptomatic carriage of Haemophilus ducreyi confirmed by the polymerase chain reaction

S Hawkes  1 , B West, S Wilson, H Whittle, D Mabey
Affiliations

Asymptomatic carriage of Haemophilus ducreyi confirmed by the polymerase chain reaction

S Hawkes et al. Genitourin Med. 1995 Aug.

Abstract

Objective: To investigate asymptomatic carriage of Haemophilus ducreyi by using polymerase chain reaction (PCR) on samples from women at high risk of infection.

Subjects: 213 commercial sex workers (CSWs) recruited in The Gambia, West Africa.

Methods: Genital samples (cervical, vaginal and ulcer) were tested for the presence of H ducreyi by PCR with the technique of "one tube nested primer".

Results: 12 CSWs were PCR positive for H ducreyi; 8 of these women had genital ulcers on examination.

Conclusion: Using a simplified PCR technique for detecting H ducreyi we have shown that 2% of CSWs were carrying the organism without clinical symptoms or signs. This has important implications for sexually transmitted disease control programmes in areas with a high prevalence of chancroid.

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