Quantitative analysis of intraepithelial large granular lymphocyte distribution and maternofetal cellular interactions in the synepitheliochorial placenta of the deer

Abstract

Intraepithelial lymphocytes are a constant feature of ruminant uterine epithelium in nonpregnant animals. Quantitation in 6 species of deer shows that the proportion of large granular lymphocytes (LGL) in this population increases markedly and continuously from the earliest implantation to the latest (midpregnant) stage examined. The size of individual granules also increases. Fetal trophectodermal binucleate cells are also present from the earliest stage in all deer examined and follow a usual ruminant life cycle: migrating and fusing with uterine epithelial cells to form trinucleate cells before releasing their granules to the maternal compartment and finally degenerating and being reabsorbed by the trophectodermal uninucleate cells. Deer LGLs were usually closely associated with the degranulating fetomaternal hybrid trinucleate cells but showed no ultrastructural changes themselves. This association indicates a dynamic interaction between deer LGL and trinucleate cells which could serve as one of the factors restricting the extent of trinucleate cell progression to a continuous syncytium as found in sheep and goats.