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. 1995 Nov;177(21):6301-3.
doi: 10.1128/jb.177.21.6301-6303.1995.

Instability of Rickettsia prowazekii RNA polymerase-promoter complexes

L P Aniskovitch  1 , H H Winkler
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Instability of Rickettsia prowazekii RNA polymerase-promoter complexes

L P Aniskovitch et al. J Bacteriol. 1995 Nov.

Abstract

The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.

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