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. 1994 Dec;41(10):639-44.
doi: 10.1111/j.1439-0450.1994.tb00274.x.

Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

J Goyache  1 , J A Orden, J L Blanco, A Doménech, G Suárez, E Gómez-Lucía
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Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

J Goyache et al. Zentralbl Veterinarmed B. 1994 Dec.

Abstract

Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12PO) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts.

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