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. 1995 Aug;31(2):134-7.
doi: 10.1007/BF00294290.

Genetic relationships among strains of Xylella fastidiosa from RAPD-PCR data

M R Pooler  1 , J S Hartung
Affiliations

Genetic relationships among strains of Xylella fastidiosa from RAPD-PCR data

M R Pooler et al. Curr Microbiol. 1995 Aug.

Abstract

Genetic relationships among 11 Xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic DNA (RAPD). Twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. Phenetic analysis based on a similarity matrix corresponded well with previous reports on X. fastidiosa RFLP-based similarity relationships, indicating that RAPD-PCR amplification products can be used as a reliable indicator of genetic distance in X. fastidiosa. Cladistic analysis suggests the existence of five groups of X. fastidiosa: the citrus group, the plum-elm group, the grape-ragweed group, the almond group, and the mulberry group.

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