Comparative Study
doi: 10.1016/0378-1119(94)00624-2.
Disruption of the target G-C base-pair by the HhaI methyltransferase
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- PMID: 7607483
- DOI: 10.1016/0378-1119(94)00624-2
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Comparative Study
Disruption of the target G-C base-pair by the HhaI methyltransferase
Gene.
.
Abstract
HhaI MTase binds DNA duplexes containing mismatches at the target base position with higher affinity than that observed for the canonical substrate. The stability of these MTase-DNA complexes inversely correlates with the strength of the base pair that is disrupted upon interaction. This finding may offer a general tool to detect other enzymes that flip bases out of the DNA helix.
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