Cloning and primary structure of the chiA gene from Aeromonas caviae
- PMID: 7608101
- PMCID: PMC177160
- DOI: 10.1128/jb.177.14.4187-4189.1995
Cloning and primary structure of the chiA gene from Aeromonas caviae
Abstract
The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.
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