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. 1995 May;33(5):1375-7.
doi: 10.1128/jcm.33.5.1375-1377.1995.

Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene

S T Gunzburg  1 , N G Tornieporth, L W Riley
Affiliations

Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene

S T Gunzburg et al. J Clin Microbiol. 1995 May.

Abstract

A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.

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