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. 1979 Jan 1;149(1):284-9.
doi: 10.1084/jem.149.1.284.

RNA and protein synthesis in human peripheral blood polymorphonuclear leukocytes

A Granelli-Piperno, J D Vassalli, E Reich

RNA and protein synthesis in human peripheral blood polymorphonuclear leukocytes

A Granelli-Piperno et al. J Exp Med. .

Abstract

Polymorphonuclear leukocytes purified from human peripheral blood synthesized RNA and proteins when placed in cell culture. Autoradiography of the cultured cells revealed that a majority of mature PMNs were engaged in macromolecule synthesis, and an analysis of newly synthesized proteins by SDS-polyacrylamide gel electrophoresis showed that many different polypeptide chains were synthesized by these cells. The rate of [3H]uridine incorporation and the pattern of newly synthesized proteins were modulated by Con A and glucocorticoids. These results suggest that in spite of their short lifetime and a large performed enzymatic apparatus, mature PMNs retain a substantial capacity for RNA and protein synthesis; and, further, that modulation of macromolecule synthesis forms part of the mechanism by which PMNs respond to inflammatory and anti-flammatory stimuli.

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