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. 1979 Jan 15;57(2):63-8.
doi: 10.1007/BF01491336.

[Properties of hypoxanthineguanine-phosphoribosyltransferase (HGPRTase) in a gout patient with partial deficiency of this enzyme (author's transl)]

[Article in German]
W Gröbner, N Zöllner

[Properties of hypoxanthineguanine-phosphoribosyltransferase (HGPRTase) in a gout patient with partial deficiency of this enzyme (author's transl)]

[Article in German]
W Gröbner et al. Klin Wochenschr. .

Abstract

Some physicochemical properties of HGPRTase were studied in hemolysates and fibroblasts of a gout patient with partial deficiency of this enzyme. In comparison to normal HGPRTase the mutant enzyme from erythrocytes was found to have an elevated apparent Km-value for hypoxanthine and guanine and a lower Km-value for PRPP. The patient's enzyme from erythrocytes is more stable at +4 degrees C and +80 degrees C, the enzyme from fibroblasts more labile than that of controls. The inhibition of the mutant enzyme by some purine nucleosides and -nucleotides differed from that found in controls. No evidence was shown for an inhibitor of the patient's HGPRTase from erythrocytes. Ultracentrifugation of hemolysate in a saccharose gradient revealed no difference in the sedimentation coefficient.

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References

    1. Am J Med. 1973 Aug;55(2):232-42 - PubMed
    1. Adv Exp Med Biol. 1973;41:327-31 - PubMed
    1. Anal Biochem. 1968 Aug;24(2):314-29 - PubMed
    1. Rev Eur Etud Clin Biol. 1972 Jan;17(1):72-5 - PubMed
    1. Biochem Pharmacol. 1975 Feb 1;24(3):379-84 - PubMed

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