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. 1995 May 9;6(7):969-72.
doi: 10.1097/00001756-199505090-00005.

N-terminal cysteines essential for Golgi sorting of B-50 (GAP-43) in PC12 cells

L H Aarts  1 , J A van der Linden, W J Hage, A J van Rozen, A B Oestreicher, W H Gispen, P Schotman
Affiliations

N-terminal cysteines essential for Golgi sorting of B-50 (GAP-43) in PC12 cells

L H Aarts et al. Neuroreport. .

Abstract

We have examined the subcellular distribution of the growth-associated protein B-50 (GAP-43) in pheochromocytoma (PC12) cells, using confocal microscopy. Proliferating PC12 cells contained very low levels of B-50 in the cytosol. Enhanced expression of B-50 in these cells, evoked by either nerve growth factor (NGF) treatment or transient transfection with rat B-50 cDNA, led to Golgi sorting and membrane targeting of the B-50 protein. Site directed mutagenesis of Cys3Cys4 to Ser3Gly4 in B-50 resulted in a cytosolic distribution. We conclude that Cys3, and Cys4 are essential for accumulation of B-50 both at the plasma membrane and in the Golgi apparatus of PC12 cells.

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