Dynamics of the interaction between Entamoeba histolytica and components of the immune response. I. Capping and endocytosis; influence of inhibiting and accelerating factors; variation of the expression of surface antigens

Abstract

The possible implications of cell membrane dynamics in the host--parasite relationship has been studied. Entamoeba histolytica rapidly redistributed and internalized antibodies and Con A bound to its surface. The process was dependent on temperature, cell metabolism and changes of pH in the environment. Phagocytizing amoebae displayed a higher rate of membrane perturbations, which were similarly affected by temperature, cell metabolism and pH variations. Cytochalasin B partially inhibited the redistribution whereas colchicine did not. Colchicine in combination with Cytochalasin B augmented the inhibitory effect of Cytochalasin B alone. The expression of antigens at the surface of the amoeba showed cyclic fluctuations during cell growth.