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. 1995 Aug;177(15):4553-6.
doi: 10.1128/jb.177.15.4553-4556.1995.

Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes

J R Alfano  1 , J H Ham, A Collmer
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Use of Tn5tac1 to clone a pel gene encoding a highly alkaline, asparagine-rich pectate lyase isozyme from an Erwinia chrysanthemi EC16 mutant with deletions affecting the major pectate lyase isozymes

J R Alfano et al. J Bacteriol. 1995 Aug.

Abstract

Erwinia chrysanthemi mutant CUCPB5047, delta(pelA pelE) delta(pelB pelC)::28bp delta(pelX) delta 4bp pehX::omega Cmr, was constructed, mutated with Tn5tac1, and screened for isopropyl-beta-D-thiogalactopyranoside-dependent pectate lyase (Pel) production. A Kmr SacI fragment from the hyperexpressing Pel+ mutant CUCPB5066 was cloned into Escherichia coli and sequenced. The gene identified, pelL, encodes a novel, asparagine-rich, highly alkaline enzyme that is similar in primary structure to PelX and in enzymological properties to PelE.

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References

    1. J Clin Microbiol. 1977 Oct;6(4):379-86 - PubMed
    1. Appl Environ Microbiol. 1985 Sep;50(3):615-22 - PubMed
    1. Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6468-72 - PubMed
    1. Mol Gen Genet. 1987 Sep;209(2):319-25 - PubMed

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