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Comparative Study
. 1995 Aug;177(16):4813-6.
doi: 10.1128/jb.177.16.4813-4816.1995.

Bacillus subtilis gnt repressor mutants that diminish gluconate-binding ability

K Yoshida  1 , H Ohmori, Y Miwa, Y Fujita
Affiliations
Comparative Study

Bacillus subtilis gnt repressor mutants that diminish gluconate-binding ability

K Yoshida et al. J Bacteriol. 1995 Aug.

Abstract

The Bacillus subtilis gnt operon is negatively regulated by GntR, which is antagonized by gluconate. Three GntR mutants with diminished gluconate-binding ability were obtained. Two were missense mutants (Met-209 to Ile and Ser-230 to Leu), whereas the third had a deletion of the C-terminal 23 amino acids. The mutant GntR proteins were unable to become properly detached from the gnt operator even in the presence of gluconate.

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