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. 1995 May;61(5):2027-9.
doi: 10.1128/aem.61.5.2027-2029.1995.

Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water

S J Park  1 , E J Lee, D H Lee, S H Lee, S J Kim
Affiliations

Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water

S J Park et al. Appl Environ Microbiol. 1995 May.

Abstract

With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media containing 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide, respectively. These two kinds of threshold units were equivalent to known concentrations of free 4-methylumbelliferone of 2.5 and 6 microM, respectively, in lauryl tryptose broth media.

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