Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1995 Jun;33(6):1640-2.
doi: 10.1128/jcm.33.6.1640-1642.1995.

Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51

B J Bricker  1 , S M Halling
Affiliations
Comparative Study

Enhancement of the Brucella AMOS PCR assay for differentiation of Brucella abortus vaccine strains S19 and RB51

B J Bricker et al. J Clin Microbiol. 1995 Jun.

Abstract

Because the brucellosis eradication program uses slaughter and quarantine as control measures, it would benefit from faster methods of bacterial identification. Distinguishing vaccine strains from strains that cause infections among vaccinated herds in the field is essential. To accomplish this, our PCR-based, species-specific assay (B. J. Bricker and S. M. Halling, J. Clin. Microbiol. 32:2660-2666, 1994) was updated to identify Brucella abortus vaccine strains S19 and RB51. Three new oligonucleotide primers were added to the five-primer multiplex Brucella AMOS PCR assay. Identification is based on the number and sizes of six products amplified by PCR.

PubMed Disclaimer

References

    1. J Bacteriol. 1990 Dec;172(12):6637-40 - PubMed
    1. Vet Microbiol. 1991 Jul;28(2):171-88 - PubMed
    1. Res Vet Sci. 1992 Sep;53(2):179-83 - PubMed
    1. Gene. 1993 Oct 29;133(1):123-7 - PubMed
    1. J Infect Dis. 1965 Jun;115:312-20 - PubMed

Publication types

LinkOut - more resources