[Establishment and characteristics of a model of nude mouse xenograft of human retinoblastoma]

Abstract

The authors reported the establishment and biological characteristics of a model of nude mouse xenograft of human retinoblastoma (RB). Immediately after the enucleation of the human globe with RB, the tumor tissue was transplanted subcutaneously into a nude mouse. The tumor grew constantly and was passaged serially for 14 passages till now. The results of the following examinations of the xenograft and primary tumor were compared: light and electron microscopy, immunohistochemistry, flow cytometry and chromosome analysis. The comparisons reveal that the main biological characteristics of the xenograft model are consistent with those of the primary tumor and also maintain throughout the serial passages. The model can be used for further laboratory studies.