[Cloning fragments of cDNA coding variable segments of light and heavy chains of monoclonal antibodies against human interleukin-2]

Abstract

DNA fragments coding for the variable fragments of the heavy and light chains of the immunoglobulin G1 against human recombinant interleukin-2 were produced using reverse transcription of the total RNA isolated from the murine hybrid myeloma cell line LNKB-2 followed by amplification of the RNA-DNA duplexes with degenerated primers. The fragments obtained were cloned into the plasmid pGEM7-Zf(+) and their structures were determined. The fragments cloned were proved to encode the Fv-fragments by sequencing N-termini of the light and heavy chains of the antibody.