rDNA targeted oligonucleotide primers for the identification of pathogenic yeasts in a polymerase chain reaction

Abstract

Species-specific oligonucleotide primers were designed for PCR identification of the basidiomycetous yeasts Cryptococcus neoformans, Trichosporon cutaneum and Rhodotorula mucilaginosa. The procedure uses standard PCR components including DNA from the test species and three primers: two universal external (upstream and downstream) limiting primers and a species-specific internal primer. Species identification requires the formation of a species-specific rDNA nucleotide segment that is significantly smaller (approximately 200 bp) than a non-target segment (approximately 600 bp). The procedure can be used to identify yeasts from single and mixed populations.