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. 1995 Jul;33(7):1948-9.
doi: 10.1128/jcm.33.7.1948-1949.1995.

Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples

M de Oña  1 , S Melón, P de la Iglesia, F Hidalgo, A F Verdugo
Affiliations

Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples

M de Oña et al. J Clin Microbiol. 1995 Jul.

Abstract

Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used for this purpose but one frequently used in clinical virology laboratories. Both cell preparations were treated with trypsin. Influenza virus was recovered from 15% of the samples inoculated in MDCK cells and from 18% of those inoculated in MRC-5 cells. The use of MRC-5 cells can simplify the search for respiratory viruses and would assist in the rapid detection of influenza virus during new epidemics.

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