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. 1995 Aug 25;23(16):3126-31.
doi: 10.1093/nar/23.16.3126.

Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

K Tang  1 , D Fu, S Kötter, R J Cotter, C R Cantor, H Köster
Affiliations

Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

K Tang et al. Nucleic Acids Res. .

Abstract

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

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