Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jul;59(7):1204-7.
doi: 10.1271/bbb.59.1204.

Nucleotide sequence of the gene for a thermostable esterase from Pseudomonas putida MR-2068

E Ozaki  1 , A Sakimae, R Numazawa
Affiliations
Free article

Nucleotide sequence of the gene for a thermostable esterase from Pseudomonas putida MR-2068

E Ozaki et al. Biosci Biotechnol Biochem. 1995 Jul.
Free article

Abstract

The esterase gene (est) of Pseudomonas putida MR-2068 was cloned into Escherichia coli JM109. An 8-kb inserted DNA directed synthesis of an esterase in E. coli. The esterase gene was in a 1.1-kb PstI-ClaI fragment within the insert DNA. The complete nucleotides of the DNA fragment containing the esterase gene were sequenced and found to include a single open reading frame of 828 bp coding for a protein of 276 amino acid residues. The open reading frame was confirmed by N-terminal amino acid sequence analysis of the purified esterase. A potential Shine-Dalgarno sequence is followed by the open reading frame. The esterase activity of the recombinant E. coli was more than 200 times higher than that of parental strain, P. putida MR-2068.

PubMed Disclaimer

Associated data