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Comparative Study
. 1995 Jul;140(3):965-72.
doi: 10.1093/genetics/140.3.965.

DNA synthesis errors associated with double-strand-break repair

J N Strathern  1 , B K Shafer, C B McGill
Affiliations
Comparative Study

DNA synthesis errors associated with double-strand-break repair

J N Strathern et al. Genetics. 1995 Jul.

Abstract

Repair of a site-specific double-strand DNA break (DSB) resulted in increased reversion frequency for a nearby allele. Site-specific DSBs were introduced into the genome of Saccharomyces cerevisiae by the endonuclease encoded by the HO gene. Expression of the HO gene from a galactose-inducible promoter allowed efficient DNA cleavage at a single site in large populations of cells. To determine whether the DNA synthesis associated with repair of DSBs has a higher error rate than that associated with genome duplication, HO-induced DSBs were generated 0.3 kb from revertible alleles of trp1. The reversion rate of the trp1 alleles was approximately 100-fold higher among cells that had experienced an HO cut than among uninduced cells. The reverted allele was found predominantly on the chromosome that experienced the DNA cleavage.

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