A novel method to study the hyphal phase of Candida albicans and to evaluate its hydrophobicity

Abstract

As little is known about the surface properties of the hyphal phase of the dimorphic fungus Candida albicans due to the difficulty in examining the latter phase in isolation, a novel method was designed to compartmentalize the two phases of the yeast using a commercially available filter-type device-Chemotaxicell. When yeast cells in the blastospore phase were incubated in filter chambers of Chemotaxicell submerged in hyphal-induction media, hyphae traversed through the pores of the filter into the exterior of the chamber enmeshing the entire outer filter surface, after 72 h of incubation. However, the inner or the chamber surface of the filter comprised mainly blastospores. The hydrophobicity of the two morphologic phases of the yeast was then compared using this method, by contact angle measurement. When the 72-h specimens with matted hyphal elements were evaluated, the contact angles varied depending on the incubation medium (such as TC 199 or serum), and in each case hydrophobicity of the hyphal phase was significantly higher than the blastopore phase yeasts. This simple, reproducible method should help not only in evaluating the properties of the hyphal elements of C. albicans but also in studying parameters such as saliva and serum, which are known to affect hyphal formation in vivo.