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Comparative Study
. 1994;35(4):389-94.
doi: 10.1186/BF03548313.

Sensitivity of certain porcine and bovine mycoplasmas to antimicrobial agents in a liquid medium test compared to a disc assay

N F Friis  1 , J Szancer
Affiliations
Comparative Study

Sensitivity of certain porcine and bovine mycoplasmas to antimicrobial agents in a liquid medium test compared to a disc assay

N F Friis et al. Acta Vet Scand. 1994.

Abstract
in English, German

The sensitivity of some porcine and bovine mycoplasmas to potent antimicrobial agents was examined. Minimal inhibitory concentration (MIC) values were estimated for M. hyosynoviae, M. hyopneumoniae, M. dispar and M. bovis against enrofloxacin, lincomycin, tetracycline, tiamulin and tylosin, in a liquid medium test and in a disc assay. All 6 examined strains of each species and the respective type strains were significantly inhibited. The greatest sensitivity was noted for tiamulin against strains of M. hyosynoviae with a final MIC50 broth value of 0.025 micrograms ml-1 and disc value of 0.03 microgram per disc. Enrofloxacin was found very potent against M. hyopneumoniae with a final MIC50 of 0.025 microgram ml-1 and 0.1 microgram per disc, and for M. dispar with 0.05 microgram ml-1 and 0.03 microgram per disc. Most disc assay estimates in micrograms per disc were similar to or moderately greater than corresponding final broth figures in microgram ml-1. It may be possible to convert observed disc assay values into representative final broth MIC values for use in the clinic.

Nogle vigtige svine- og kvægmykoplasmer blev undersøgt for følsomhed over for specielt udvalgte, potente antimikrobielle forbindelser. Den laveste hæmmende koncentration (MIC værdi) blev bestemt for 6 nye isolater samt typestammen af de porcine arter, M. hyosynoviae og M. hyopneumoniae, samt de bovine arter M. dispar og M. bovis, over for enrofloxacin, lincomycin, tetracyclin, tiamulin og tylosin. Der blev anvendt både en test i flydende medium med tidlig og slut MIC registrering samt en disk metode.

Alle isolater samt typestammerne fandtes tydeligt følsomme under begge forsøgsbetingelser. Den største følsomhed blev registreret for tiamulin over for M. hyosynoviae, hvor der fandtes en slut MIC50 værdi på 0.025 μg pr. ml og en disk værdi på 0.03 μg pr. disk. Også enrofloxacin fandtes bemærkelsesværdigt aktivt over for M. hyopneumoniae med slut MIC50 på 0.025 μg pr. ml og 0.1 μg pr. disk og over for M. dispar på 0.05 μg pr. ml og 0.03 μg pr. disk. I forsøg med enrofloxacin, lincomycin og tiamulin fandtes kun mindre stammevariation inden for mykoplasmaarterne, medens spredningen var noget større for tetracyclin og tylosin; en forskel, som kunne afspejle intensiteten i brugen over årene.

I de fleste forsøg fandtes disk værdierne i μg pr. disk på niveau med eller moderat højere end de tilsvarende slutværdier i μg pr. ml. Det synes derfor muligt at konvertere observerede disk assay værdier til repræsentative slutværdier i μg pr. ml ved hjælp af en simpel omregningsfaktor med henblik på udnyttelse i klinikken.

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