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. 1993 Mar;59(3):915-8.
doi: 10.1128/aem.59.3.915-918.1993.

Direct extraction and purification of rRNA for ecological studies

M A Moran  1 , V L Torsvik, T Torsvik, R E Hodson
Affiliations

Direct extraction and purification of rRNA for ecological studies

M A Moran et al. Appl Environ Microbiol. 1993 Mar.

Abstract

Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.

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References

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