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. 1976 Mar 18;260(5548):221-5.
doi: 10.1038/260221a0.

Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA

T H Rabbitts

Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA

T H Rabbitts. Nature. .

Abstract

DNA copies of rabbit globin mRNA have been inserted into the plasmid mini-ColEl. After addition of poly(dT) tails, the plasmid was used to prime the copying of mRNA by reverse transcriptase. Mini-ColEl carrying globin sequences were denatured and annealed with mini-ColEl tailed with poly(dA). Functional plasmids containing the synthesised globin sequences were isolated by transfection in E. coli.

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