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Comparative Study
. 1993 Aug 15;90(16):7598-602.
doi: 10.1073/pnas.90.16.7598.

Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons

K Goto  1 , H Kondo
Affiliations
Comparative Study

Molecular cloning and expression of a 90-kDa diacylglycerol kinase that predominantly localizes in neurons

K Goto et al. Proc Natl Acad Sci U S A. .

Abstract

A diacylglycerol kinase cDNA was isolated from a rat brain cDNA library. This cDNA encoded an 801-amino acid protein of 90,287 Da. This 90-kDa diacylglycerol kinase showed 58% identity in deduced amino acid sequence with a previously isolated rat 80-kDa diacylglycerol kinase. EF-hand motifs, cysteine-rich zinc-finger-like sequences, and putative ATP-binding sites were all conserved between the two kinase species. However, mRNA encoding the 90-kDa kinase was confined to restricted neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle. Further, the 90-kDa kinase was found to exhibit high phosphorylation activity for long-chain diacylglycerols and was mainly associated with the membrane fraction when the cDNA was transfected into COS-7 cells.

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