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. 1976 Feb;3(2):343-53.
doi: 10.1093/nar/3.2.343.

The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii

D I Smith, F R Blattner, J Davies
Free PMC article

The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii

D I Smith et al. Nucleic Acids Res. 1976 Feb.
Free PMC article

Abstract

We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and requirements. Pst 1 requires Mg++ as the only cofactor and produces more than 18 cleavages in wild type lambda. We have determined the location of 7 of these cleavages by the use of deletion and insertion mutants of lambda.

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References

    1. Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7 - PubMed
    1. Arch Biochem Biophys. 1968 Jul;126(1):155-64 - PubMed
    1. J Virol. 1974 Nov;14(5):1235-44 - PubMed
    1. J Mol Biol. 1971 Mar 14;56(2):369-84 - PubMed
    1. J Biol Chem. 1965 Sep;240(9):3685-92 - PubMed

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