[ELISA test for granulocyte colony stimulating factor using monoclonal antibody and its clinical results]

Abstract

Granulocyte colony stimulating factor (G-CSF) level in the serum rises within minutes after people infected by bacteria. It can reach almost 1,000 fold above normal level. We established an ELISA test for measuring G-CSF using McAb. After injected with endotoxin into mice, this method was used to observe the peak serum G-CSF in mouse. Again 200 normal people were tested on G-CSF, but all showed negative results. In 100 bacteria-infected patients, 82 (82%) showed positive results. In 100 non-bacteria-infected patients with fever, only 7(7%) was positive. It is indicated that testing body fluids and serum can determine whether the patient is infected with bacteria or not.