Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Sep 15;90(18):8392-6.
doi: 10.1073/pnas.90.18.8392.

Production of high-titer helper-free retroviruses by transient transfection

W S Pear  1 , G P Nolan, M L Scott, D Baltimore
Affiliations

Production of high-titer helper-free retroviruses by transient transfection

W S Pear et al. Proc Natl Acad Sci U S A. .

Abstract

The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. The resulting ecotropic virus packaging cell line BOSC 23 produces infectious retrovirus at > 10(6) infectious units/ml of supernatant within 72 hr after CaPO4-mediated transfection. A stringent assay for replication-competent virus showed that no helper virus was present. The system can produce high titers of retroviral vectors expressing genes that are extremely difficult to propagate at high titer in stable producer lines. This method should facilitate and extend the use of helper-free retroviral gene transfer, as well as be useful for gene therapy.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1989 Mar;9(3):1263-70 - PubMed
    1. Immunol Today. 1989 Oct;10(10):344-50 - PubMed
    1. Science. 1990 Feb 16;247(4944):824-30 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6506-10 - PubMed
    1. J Virol. 1992 Aug;66(8):5110-3 - PubMed

Publication types

MeSH terms