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. 1976 Mar 25;428(1):182-92.
doi: 10.1016/0304-4165(76)90119-7.

A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae

A Toh-E, H Nakamura, Y Oshima

A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae

A Toh-E et al. Biochim Biophys Acta. .

Abstract

Recessive mutants defective in the formation of non-specific alkaline phosphatase (EC 3.1.3.1) could be selected by staining colonies on a plate with p-nitrophenylphosphate after treatment with chloroform vapour. Since no complementation was observed among the nine mutants so far tested, all the mutations might occur in the same locus, phoH. The non-specific alkaline phosphatase was repressible, although a significant basal level of the enzyme activity was observed in the repressed condition.

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