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. 1995 Mar 21;155(1):129-30.
doi: 10.1016/0378-1119(94)00711-z.

SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'

T G Simcox  1 , L Fabian, K Kretz, V Hedden, M E Simcox
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SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'

T G Simcox et al. Gene. .

Abstract

A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.

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