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. 1995 Mar 21;155(1):133-4.
doi: 10.1016/0378-1119(94)00909-c.

New vectors for the in vitro generation of alkaline phosphatase fusions to proteins encoded by G+C-rich DNA

K E Mdluli  1 , J D Treit, V J Kerr, F E Nano
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New vectors for the in vitro generation of alkaline phosphatase fusions to proteins encoded by G+C-rich DNA

K E Mdluli et al. Gene. .

Abstract

Phagemid vectors were constructed to allow fusions of alkaline phosphatase to proteins encoded by G+C-rich DNA, by engineering a BstBI site (TT/CGAA) in front of a phoA gene that lacks an encoded signal peptide. Three vectors (pJDT1, pJDT2 and pJDT3), each with phoA in a different reading frame with respect to the BstBI site, were produced; a lacP region is present in each plasmid upstream of the BstBI site. The presence of the BstBI site allows the random cloning of G+C-rich DNA digested with a number of restriction enzymes that generate cohesive ends.

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