Cloning and primary sequence of the rpoH gene from Pseudomonas aeruginosa
- PMID: 7698670
- DOI: 10.1016/0378-1119(94)00829-h
Cloning and primary sequence of the rpoH gene from Pseudomonas aeruginosa
Abstract
A DNA fragment from Pseudomonas aeruginosa containing the rpoH gene encoding the heat-shock sigma factor sigma 32 has been cloned and sequenced. The gene is expressed in Escherichia coli and complements an rpoH- strain. An open reading frame encoding 284 amino acids shows 61% identity and 78% similarity to the RpoH protein of Escherichia coli.
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