Escherichia coli mutants altered in murein lipoprotein
- PMID: 770420
- PMCID: PMC233269
- DOI: 10.1128/jb.126.1.147-156.1976
Escherichia coli mutants altered in murein lipoprotein
Abstract
Mutants with alterations in the structure, biosynthesis, or assembly of murein lipoprotein were selected by a procedure based on radiation suicide of wild-type organisms by [3H]arginine under conditions where the radioactive arginine was preferentially incorporated into lipoprotein. Further screening for the potential mutants among the survivors of [3H]arginine suicide was carried out by using a sensitive immunodiffusion test, followed by radioactive double-labeling experiments. Three mutants were obtained and partially characterized.
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