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. 1976 Apr;126(1):147-56.
doi: 10.1128/jb.126.1.147-156.1976.

Escherichia coli mutants altered in murein lipoprotein

Escherichia coli mutants altered in murein lipoprotein

H C Wu et al. J Bacteriol. 1976 Apr.

Abstract

Mutants with alterations in the structure, biosynthesis, or assembly of murein lipoprotein were selected by a procedure based on radiation suicide of wild-type organisms by [3H]arginine under conditions where the radioactive arginine was preferentially incorporated into lipoprotein. Further screening for the potential mutants among the survivors of [3H]arginine suicide was carried out by using a sensitive immunodiffusion test, followed by radioactive double-labeling experiments. Three mutants were obtained and partially characterized.

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