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. 1995 Mar 28;92(7):2474-8.
doi: 10.1073/pnas.92.7.2474.

Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells

I M Robinson  1 , J M Finnegan, J R Monck, R M Wightman, J M Fernandez
Affiliations

Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells

I M Robinson et al. Proc Natl Acad Sci U S A. .

Abstract

"Snapshot" images of localized Ca2+ influx into patch-clamped chromaffin cells were captured by using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca2+ that had the appearance of either "hotspots" or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were colocalized. These results provide functional support for the idea that secretion occurs from "active zone"-like structures in neuroendocrine cells.

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