The rapid assembly of synaptic sites in photoreceptor terminals of the fly's optic lobe recovering from cold shock

Abstract

When a housefly, Musca domestica, is subject to cold exposure (0 degrees C for 24 hr), a number of obvious changes are seen in the first optic neuropil, or lamina, beneath the compound eye. In particular, the number of afferent photoreceptor synapses declines by about 30%. This loss is dramatically restored after warm recovery at 23 degrees C for 24 hr. Synapses disappear at an average rate of 2-3/hr during cold exposure and reappear at a maximal rate of more than 20/hr during the first 2 hr of warm recovery. Thereafter their number temporarily overshoots control values, to increase at 6 hr of warm recovery to 60% above their cold-exposed minimum. The number subsequently returns more or less to normal. These changes demonstrate the lability of synaptic sites under these conditions, with individual sites forming and disappearing rapidly. The changes also interrupt the close correlation between synaptic number and the surface area of the receptor terminal, a correlation that normally conserves synaptic spacing density. The density is preserved during cold exposure but increases during warm recovery at a time when the addition of newly formed synapses exceeds the slower increase in receptor terminal size.