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Comparative Study
. 1995 Apr 5;1248(1):75-9.
doi: 10.1016/0167-4838(95)00007-h.

Affinity-purification and identification of GrpE homologues from mammalian mitochondria

D J Naylor  1 , M T Ryan, R Condron, N J Hoogenraad, P B Høj
Affiliations
Comparative Study

Affinity-purification and identification of GrpE homologues from mammalian mitochondria

D J Naylor et al. Biochim Biophys Acta. .

Abstract

We used affinity chromatography on DnaK columns to identify a mitochondrial GrpE homologue from bovine, porcine and rat liver mitochondria. The 24 kDa GrpE homologue bound specifically to the DnaK column and was not eluted with 1 M KCl but readily with 5 mM ATP. Sequence analysis of the bovine homologue (85 residues) revealed 42% positional identity to mitochondrial GrpEp from S. cerevisiae and about 30% identity to the bacterial counterparts. Thus, GrpE homologues from higher and lower eukaryotes are highly conserved.

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