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Comparative Study
. 1995 Apr 4;1261(2):296-300.
doi: 10.1016/0167-4781(95)00030-k.

Xenopus PKN: cloning and sequencing of the cDNA and identification of conserved domains

H Mukai  1 , K Mori, H Takanaga, M Kitagawa, H Shibata, M Shimakawa, M Miyahara, Y Ono
Affiliations
Comparative Study

Xenopus PKN: cloning and sequencing of the cDNA and identification of conserved domains

H Mukai et al. Biochim Biophys Acta. .

Abstract

cDNA clone encoding Xenopus laevis PKN has been isolated from Xenopus kidney library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 901 amino acids. Immunoprecipitate from cytoplasmic fraction of COS7 cells transfected with this cDNA construct using antiserum against bacterially expressed Xenopus PKN revealed arachidonic acid-dependent autophosphorylation activity. Comparison of the closely related sequences of human and rat PKN with a protein from evolutionarily distant Xenopus, revealed several highly invariant domains in the NH2-terminal regulatory regions, suggesting that they participate in binding interaction with arachidonic acid.

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