Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Dec;86(3):341-6.

Anti-lipid A monoclonal antibodies and anti-LPS antiserum effects on limulus activity of LPS

M Goto  1 , T Yoshioka, R Lichtenberg, C H Lee, W P Zeller
Affiliations
  • PMID: 7712110

Anti-lipid A monoclonal antibodies and anti-LPS antiserum effects on limulus activity of LPS

M Goto et al. Res Commun Mol Pathol Pharmacol. 1994 Dec.

Abstract

Anti-lipid A monoclonal antibodies (A78S1 and A523) and anti-LPS antiserum can decrease the mortality due to endotoxic shock in the newborn rat. However, in vitro LPS detoxification of anti-lipid A monoclonal antibodies is not known. Thus, we studied in vivo effects of A78S1 (IgG), A523 (IgM), and anti-LPS antiserum on Limulus activity. Anti-LPS antiserum decreased Limulus activity of S. enteritidis, E. coli and S. typhosa LPS. However, neither A78S1 nor A523 decreased the Limulus activity of E. coli and S. typhosa LPS. A78S1 or A523 incubation with S. enteritidis LPS at different doses (0.1 to 100 ng/ml) did not alter the Limulus activity. Perchloric acid treatment after LPS incubation with A78S1 or A523 significantly decreased Limulus activity. Therefore, antilipid A monoclonal antibodies (A78S1 and A523) can bind LPS, but do not decrease Limulus activity.

PubMed Disclaimer

Publication types