Crystallization and preliminary structural studies of the ncd motor domain
- PMID: 7716170
- DOI: 10.1002/prot.340210108
Crystallization and preliminary structural studies of the ncd motor domain
Abstract
The motor domain of the kinesin homolog ncd has been crystallized in the presence of MgATP by the vapor diffusion method using polyethylene glycol as the precipitant. The crystals belong to the orthorhombic space group I222 with unit cell dimensions a = 127.1 A, b = 122.3 A, c = 68.0 A, and there is one ncd molecule per asymmetric unit. The crystals diffract X-ray to at least 2.3 A and are appropriate for high-resolution structure determination.
Similar articles
-
Crystallization and preliminary X-ray analysis of the single-headed and double-headed motor protein kinesin.J Struct Biol. 1997 Jun;119(1):28-34. doi: 10.1006/jsbi.1997.3872. J Struct Biol. 1997. PMID: 9216086
-
Crystal structure of the motor domain of the kinesin-related motor ncd.Nature. 1996 Apr 11;380(6574):555-9. doi: 10.1038/380555a0. Nature. 1996. PMID: 8606780
-
Crystallization and preliminary X-ray diffraction studies of curculin. A new type of sweet protein having taste-modifying action.J Mol Biol. 1994 Apr 29;238(2):286-7. doi: 10.1006/jmbi.1994.1289. J Mol Biol. 1994. PMID: 8158656
-
Kinesin and NCD, two structural cousins of myosin.J Muscle Res Cell Motil. 1996 Apr;17(2):173-5. doi: 10.1007/BF00124239. J Muscle Res Cell Motil. 1996. PMID: 8793719 Review. No abstract available.
-
Crystallization of and preliminary crystallographic data for the N-terminal half-molecule of ovotransferrin.J Biochem. 1990 Dec;108(6):907-8. doi: 10.1093/oxfordjournals.jbchem.a123312. J Biochem. 1990. PMID: 2089038 Review.
Cited by
-
The shapes of the motor domains of two oppositely directed microtubule motors, ncd and kinesin: a neutron scattering study.Biophys J. 1995 Oct;69(4):1563-8. doi: 10.1016/S0006-3495(95)80028-7. Biophys J. 1995. PMID: 8534827 Free PMC article.
-
A novel adenosine triphosphate analog with a heavy atom to target the nucleotide binding site of proteins.Protein Sci. 1995 Sep;4(9):1824-31. doi: 10.1002/pro.5560040917. Protein Sci. 1995. PMID: 8528080 Free PMC article.
-
Nucleotide-dependent movements of the kinesin motor domain predicted by simulated annealing.Biophys J. 1998 Aug;75(2):646-61. doi: 10.1016/S0006-3495(98)77555-1. Biophys J. 1998. PMID: 9675167 Free PMC article.
-
Polarity of microtubule assemblies during neuronal cell migration.Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9218-22. doi: 10.1073/pnas.93.17.9218. Proc Natl Acad Sci U S A. 1996. PMID: 8799181 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources