[Epoxy embedding of bone-marrow biopsies for light microscopy and histopathologic use (author's transl)]

Abstract

A routine method of epoxy embedding of bone-marrow and other tissues for light microscopy is described. Undecalcified bone specimens obtained from the sternum or iliac crest using trephine biopsy are fixed in formalin, deshydrated in acetone, embedded in a Kushida's low-viscosity epoxy resin medium. The samples are cut using a carbide-edged knife in a rotary microtome or a glass knife in a Sorval JB4 microtome. After fixing sections to slide with Neoprene and removal of the embedding medium with sodium methoxide and acetone, the staining with usual histological procedures is quite easy. A simple and rapid technique is outlined for staining these sections with Giemsa. The resolution, compared with routine parafin sections, is improved.